Bioreactor Fermenter

Material

Wetted parts are SS316L, and others is SS304.

Surface finish

inside surface is 0.28-0.4μm by mechanical polish, ( passivation or electro-polish are optional )
outside surface is 0.4-0.8μm by satin finish.

Pressure

Working pressure inside vessel:0.2mpa
Design pressure inside vessel: 0.25mpa
Working pressure inside jacket:0.3mpa
Design pressure inside jacket: 0.35mpa
Pressure can be also designed according to client’s detail requirement.

Agitation

1.Fermentor use SEW decelerator
2.top mechanical agitator with two layer six blade impeller, one layer defoamer and four baffles.
3.mechanical seal (single or double mechanical seal)
4.speed range: 50-400rpm (it depends on the size of the vessel)

Equips of vessel

1.using alcohol flame inoculation
2.sterile sampling vale and bottom valve
3.PH, DO, temp. sensor on lower side, side glass, top with foam and level probe, and 12v sight glass with lamp, etc.

Construction

Floor-type
Three fermentor vessels or more than three ferementors with one control panel (simens PLC and HMI)
They can work independently and can work together at same time.

Operating panel

Chinese and English version

Main components and controls of system

PH

1.On-line check and measure, and digitization fully automatic control;
2.PH, acid and base gang control. Peristaltic pump automatically adjust to feed acid and base;
3.Control range 2-12pH, measure precision±0.01 pH, control precision±0.1 pH;
4.It has auto alarm function.
5.pH sensor and signal transmission line, Mettler toledo

DO

1.On-line check and measure, cascade with rpm; digital display.
2.Range 0-100% or 0-200%, precision ±1%;
3.It has auto alarm function;
4.DO sensor and signal transmission line, Mettler toledo

Foam control

1.Automatically control and automatically alarm;
2. Stainless steel electrode check, peristaltic pump automatically add defoamer.

Feed control

1. Automatically control and automatically alarm;
2.Stainless steel electrode check, peristaltic pump automatically feeding

Temp. control

1.PID fully digitization signal processing, digitization control settings
2.Circulating water automatically control temperature
3.Measure range 0-150°C, control range 15-45°C,control precision±0.2°C
4.Pt 100Ωtemp. Sensor
5.system with electrical heater to control the temp inside vessel
6.water pump circulation
7.solenoid valve to control

Rotation speed

1. Automatically control , with auto alarm function
2. Siemens frequency converter to adjust the speed.

Pressure

1.Manually adjusting with pressure gauge and manual valve (standard system)
2.Automatically adjusting with pressure transmitter and PID valve and pneumatic valves (optional)

Air flow

1.Manually adjusting with rotor meter and manual valve (standard system)
2.Automatically adjusting with mass-flow meter and pneumatic valves (optional)
3.Air in filter , tail gas filter , with PALL cartridge, precision 0.2μm

Sterilization

1.It can be SIP by connecting with steam
2.Manually control with manual valve (standard one)
3.Automatic control with pneumatic valves (optional)

Control

Siemens PLC, and Siemens 10.4” LCD touch screen(HMI) and RITAI program

PC

1.PC with Kingview software for recording function (optional)
2.PC with Siemens remote control and Kingview recording function (optional)
3.printer (optional)